Preparation, characterization, immobilization, and molecular docking analysis of a novel detergent-stable subtilisin-like serine protease from Streptomyces mutabilis …

S Mechri, F Allala, K Bouacem, I Hasnaoui… - International Journal of …, 2022 - Elsevier
… 2A) with maximum changes in the secondary structure. The sodium alginate spectrum …
Accordingly, the hydrophobic interactions and van der Waals interactions may reinforce this …

Improving the alkaline stability of pepsin through rational protein design using renin, an alkaline-stable aspartic protease, as a structural and functional reference

DAS Grahame, JH Dupuis, BC Bryksa, T Tanaka… - Enzyme and Microbial …, 2021 - Elsevier
… X 2 motif where X 1 is a hydrophobic amino acid and X 2 is Ser or … based upon their secondary
structural features and … to the proteins surface charge can increase its thermal stability [54]…

Stabilization and improved properties of Salipaludibacillus agaradhaerens alkaline protease by immobilization onto double mesoporous core-shell nanospheres

ASS Ibrahim, YB Elbadawi, AM El-Toni… - International Journal of …, 2021 - Elsevier
… work, a recently characterized alkaline serine protease from … -R alkaline protease through
the spacer group can enhance the … /hydrophobic interaction between the AK-R protease

Molecular dynamics studies of disulfide bonds for enhancing the stability of serine protease PB92

G Zhao, J Zhang - New Journal of Chemistry, 2024 - pubs.rsc.org
… various factors, including hydrophobic interactions, hydrogen … bonds into protease PB92 to
enhance its stability. Recognizing … changes in the secondary structure of the protease and its …

[HTML][HTML] Cloning, expression, and structural elucidation of a biotechnologically potential alkaline serine protease from a newly isolated haloalkaliphilic Bacillus …

HB Bhatt, SP Singh - Frontiers in microbiology, 2020 - frontiersin.org
… Primary and secondary structure properties of the protease and … hydrogen bonds and
hydrophobic interactions depriving the … The negative charges enhance protein solubility through …

[HTML][HTML] Improving the Thermostability of Serine Protease PB92 from Bacillus alcalophilus via Site-Directed Mutagenesis Based on Semi-Rational Design

H Miao, X Xiang, N Han, Q Wu, Z Huang - Foods, 2023 - mdpi.com
… of the protein structure, the secondary structure is mostly … proteins, as these residues
enhance the hydrophobicity and … In our investigation, the additional hydrophobic interactions

Thermostability engineering of industrial enzymes through structure modification

NG Nezhad, RNZRA Rahman, YM Normi… - Applied Microbiology …, 2022 - Springer
interactions through single mutant, hydrophobic interaction, … between secondary structures
and substitutions at preferable … electrostatic interactions plays a detrimental role to reinforce

Heterologous Expression and Structural Elucidation of a Highly Thermostable Alkaline Serine Protease from Haloalkaliphilic Actinobacterium, Nocardiopsis sp. Mit-7

FJ Thakrar, GA Koladiya, SP Singh - Applied Biochemistry and …, 2023 - Springer
… and Proteinase K (100 μg/ml) were also used to enhance the … followed by hydrophobic
interaction chromatography using … , including information of secondary structure, functional sites…

Improving the Activity and Stability of Serine Protease ThAPT3 by Alleviating Self-Cleavage and Its Application in Deproteinization of Shrimp Shells

X Wang, T Dong, Q Zhou, L Tong, J Zheng… - Journal of Agricultural …, 2023 - ACS Publications
… (H-bond), or hydrophobic interactions can be used to … secondary structures in the C-terminal
region (the primary autolysis region) were individually optimized to enhance the stability of …

Improved production of alkaline and solvent-stable proteases from a halotolerant Exiguobacterium isolate through heterologous expression

N Srivastava, S Shiburaj, SK Khare - International Journal of Biological …, 2024 - Elsevier
… the overexpression of detergent stable serine protease from … the effect of solvents on the
secondary structure of the proteases. … to enhance their activity and prolonged stability for their …